Here are your results
You submited 3 sequence/s. We found 7 IRE/s in 3 different sequence/s:
| Sequence | Start | End | Loop Type | Mismatches | 3' Bulge | N25 | GU/UG | Pred. Loop | Pred. Pairs | Pred. C8 | Free Energy | Quality |
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| HUMAN-SLC11A2-IRE-EN... | 1801 | 1833 | 1 | - | N22b:T | A | 1 | -6.10 | High | |||
| HUMANFTL-NM_000146 | 26 | 57 | 1 | - | - | C | 1 | -1.50 | High | |||
| mouse-TFR1-NM_011638 | 3164 | 3195 | 2 | - | - | A | 0 | -7.20 | High | |||
| mouse-TFR1-NM_011638 | 3223 | 3254 | 1 | - | - | A | 0 | -8.40 | High | |||
| mouse-TFR1-NM_011638 | 3602 | 3633 | 1 | - | - | A | 1 | -9.00 | High | |||
| mouse-TFR1-NM_011638 | 3667 | 3698 | 1 | - | - | A | 0 | -7.90 | High | |||
| mouse-TFR1-NM_011638 | 3714 | 3745 | 1 | - | - | A | 0 | -8.10 | High |
HELP: GFF Format
GFF format if you use standard sequence ids, allows you to load your results in a Genome Browser Close
Sequence ID
HUMAN-SLC11A2-IRE-ENSEMBL
Sequence Context
Position in sequence: 1801-1833
1667 TGGCAATGTTTGATTGCACTGGGCATGTCCTTCCTGGACTGTGGGCATACGGTAAGCATC 1726
1727 TCTAAAGGCCTGCTGACAGAAGAAGCCACCCGTGGCTACGTTAAATAACACTGGATTAGT 1786
1787 CTGTCTTCTGCAGGTAGCCATCAGAGCCAGTGTGTTTCTATGGTTTACTGTGTGAACATA 1846
1847 GCCAAAAGTATGTGCCGTTGCACAGACTGTGTTTATGACTCAACCGTTGGTTGGAAAAGA 1906
1907 CTTTGTTTCATGTGTATTTGAAAGATGGAATTATTTTTTCCTTCCTGACCTAACCTTAGA 1966
HELP: FASTA Format
download the IRE sequence in FASTA format if you want to use it in sequence analysis programs as BLAST.Close
Overall Quality: High
- SIREs Prediction
- RNAfold Prediction
HELP: SIRES Prediction/RNAFold Prediction
Predicted pairings are graphically displayed using our algorithm and the RNAFold predicted program (Vienna RNA Package)
Close
Report
Click on the features to learn what they mean
SIREs Prediction
Loop is of the canonical type. Motif class: 1
HELP: Motif type
There are 18 types of motives. Motifs 1 and 2 are the canonical ones, while 3 to 18 are derived from SELEX experiments. Please look at the "Docs Pages" to know more CloseNo Mismatches
HELP: Mismatch
We allow one, but only one mistmatch in the stem. The presence of a mismatch an a bulge (see the Bulge help) are incompatibleClose-
3' Bulge at postion 22b
HELP: 3' Bulge
An unpaired base (a "Bulge") is allowed at positions 20b,21b,22b or 23b. The presence of a mismatch (see the mismatches help) and a buldge are incompatibleClose -
n25 is not a G
HELP: The 25th base
A G Residue in this position could compromise the IRE stability since it could pair with the C8, which needs to be free to interact with the Iron Regulatory proteins (IRPs).Close 1 GU Pair
HELP: GU/UG Pairings
GU/UG parings are possible in RNA strands. However, experiments suggest that only 2 of those can be present in the IRE stem for it to be stable.CloseSIREs Prediction vs. RNAFold Prediction
-
Loop matches the RNAFold prediction
HELP: Predicted Loop
The 6 nucleotide apical loop is also fold as a loop in the RNAFold prediction. Close -
Pairings in the upper stem does NOT match the RNAFold prediction
HELP: Predicted Pairings
The base pairings in the upper stem predicted by SIREs are also pairing in the RNAFold prediction. Close C8 Bulge is present in the RNAFold prediction
HELP: C8 in the predicted folding
The C8 bulge predicted by SIREs is also predicted in the RNAFold structure as an unpair nucleotide. Nucleotide at position 8 (C8 bulge) is an important contact site with the IRPs. CloseFree Energy
Free Energy: -6.10 kcal/mol
HELP:Minimum Free Energy
Minimum Free Energy of the folded RNA structure has been calculated with the Vienna Package. Please visit Vienna package Site for further information.Close
Hover over the features to highlight them in the structure
Sequence ID
HUMANFTL-NM_000146
Sequence Context
Position in sequence: 26-57
1 GCAGTTCGGCG 11
12 GTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGGACGGAACAGATCCGGGGACTCTC 71
72 TTCCAGCCTCCGACCGCCCTCCGATTTCCTCTCCGCTTGCAACCTCCGGGACCATCTTCT 131
132 CGGCCATCTCCTGCTTCTGGGACCTGCCAGCACCGTTTTTGTGGTTAGCTCCTTCTTGCC 191
HELP: FASTA Format
download the IRE sequence in FASTA format if you want to use it in sequence analysis programs as BLAST.Close
Overall Quality: High
- SIREs Prediction
- RNAfold Prediction
HELP: SIRES Prediction/RNAFold Prediction
Predicted pairings are graphically displayed using our algorithm and the RNAFold predicted program (Vienna RNA Package)
Close
Report
Click on the features to learn what they mean
SIREs Prediction
Loop is of the canonical type. Motif class: 1
HELP: Motif type
There are 18 types of motives. Motifs 1 and 2 are the canonical ones, while 3 to 18 are derived from SELEX experiments. Please look at the "Docs Pages" to know more CloseNo Mismatches
HELP: Mismatch
We allow one, but only one mistmatch in the stem. The presence of a mismatch an a bulge (see the Bulge help) are incompatibleClose-
No 3' Bulges
HELP: 3' Bulge
An unpaired base (a "Bulge") is allowed at positions 20b,21b,22b or 23b. The presence of a mismatch (see the mismatches help) and a buldge are incompatibleClose -
n25 is not a G
HELP: The 25th base
A G Residue in this position could compromise the IRE stability since it could pair with the C8, which needs to be free to interact with the Iron Regulatory proteins (IRPs).Close 1 GU Pair
HELP: GU/UG Pairings
GU/UG parings are possible in RNA strands. However, experiments suggest that only 2 of those can be present in the IRE stem for it to be stable.CloseSIREs Prediction vs. RNAFold Prediction
-
Loop matches the RNAFold prediction
HELP: Predicted Loop
The 6 nucleotide apical loop is also fold as a loop in the RNAFold prediction. Close -
Pairings in the upper stem match the RNAFold prediction
HELP: Predicted Pairings
The base pairings in the upper stem predicted by SIREs are also pairing in the RNAFold prediction. Close C8 Bulge is present in the RNAFold prediction
HELP: C8 in the predicted folding
The C8 bulge predicted by SIREs is also predicted in the RNAFold structure as an unpair nucleotide. Nucleotide at position 8 (C8 bulge) is an important contact site with the IRPs. CloseFree Energy
Free Energy: -1.50 kcal/mol
HELP:Minimum Free Energy
Minimum Free Energy of the folded RNA structure has been calculated with the Vienna Package. Please visit Vienna package Site for further information.Close
Hover over the features to highlight them in the structure
Sequence ID
mouse-TFR1-NM_011638
Sequence Context
Position in sequence: 3164-3195
3030 CCAACATTTTCTGAAAAAGAACAAGTTTTAGACTCAATTTGTCAGACTTGAAAAGAACAC 3089
3090 ACTGCCAAGTTTTGGCCAAAGTGTTAGTCTTCAGGAAAGCTTTCTATCATTTTGGCACTG 3149
3150 AGATATTTATTGTTTATTTATCAGTGACAGAGTTCACTATAAATAGTGTTGTTTTTTTTT 3209
3210 TATATATAGAAGATAATTATCGGAAGCAGTGCCTTCCATAATTATGACAGTTATACTGTC 3269
3270 GTTTTCTTTTAATAAAAGCAGCATCTGCTAATGAGACCCACAGATACTGGAAGTTTTGCA 3329
HELP: FASTA Format
download the IRE sequence in FASTA format if you want to use it in sequence analysis programs as BLAST.Close
Overall Quality: High
- SIREs Prediction
- RNAfold Prediction
HELP: SIRES Prediction/RNAFold Prediction
Predicted pairings are graphically displayed using our algorithm and the RNAFold predicted program (Vienna RNA Package)
Close
Report
Click on the features to learn what they mean
SIREs Prediction
Loop is of the canonical type. Motif class: 2
HELP: Motif type
There are 18 types of motives. Motifs 1 and 2 are the canonical ones, while 3 to 18 are derived from SELEX experiments. Please look at the "Docs Pages" to know more CloseNo Mismatches
HELP: Mismatch
We allow one, but only one mistmatch in the stem. The presence of a mismatch an a bulge (see the Bulge help) are incompatibleClose-
No 3' Bulges
HELP: 3' Bulge
An unpaired base (a "Bulge") is allowed at positions 20b,21b,22b or 23b. The presence of a mismatch (see the mismatches help) and a buldge are incompatibleClose -
n25 is not a G
HELP: The 25th base
A G Residue in this position could compromise the IRE stability since it could pair with the C8, which needs to be free to interact with the Iron Regulatory proteins (IRPs).Close No GU Pairs
HELP: GU/UG Pairings
GU/UG parings are possible in RNA strands. However, experiments suggest that only 2 of those can be present in the IRE stem for it to be stable.CloseSIREs Prediction vs. RNAFold Prediction
-
Loop matches the RNAFold prediction
HELP: Predicted Loop
The 6 nucleotide apical loop is also fold as a loop in the RNAFold prediction. Close -
Pairings in the upper stem match the RNAFold prediction
HELP: Predicted Pairings
The base pairings in the upper stem predicted by SIREs are also pairing in the RNAFold prediction. Close C8 Bulge is present in the RNAFold prediction
HELP: C8 in the predicted folding
The C8 bulge predicted by SIREs is also predicted in the RNAFold structure as an unpair nucleotide. Nucleotide at position 8 (C8 bulge) is an important contact site with the IRPs. CloseFree Energy
Free Energy: -7.20 kcal/mol
HELP:Minimum Free Energy
Minimum Free Energy of the folded RNA structure has been calculated with the Vienna Package. Please visit Vienna package Site for further information.Close
Hover over the features to highlight them in the structure
Sequence ID
mouse-TFR1-NM_011638
Sequence Context
Position in sequence: 3223-3254
3089 CACTGCCAAGTTTTGGCCAAAGTGTTAGTCTTCAGGAAAGCTTTCTATCATTTTGGCACT 3148
3149 GAGATATTTATTGTTTATTTATCAGTGACAGAGTTCACTATAAATAGTGTTGTTTTTTTT 3208
3209 TTATATATAGAAGATAATTATCGGAAGCAGTGCCTTCCATAATTATGACAGTTATACTGT 3268
3269 CGTTTTCTTTTAATAAAAGCAGCATCTGCTAATGAGACCCACAGATACTGGAAGTTTTGC 3328
3329 ACTTATGGTCAGCACTTGCAACTTTAGGAAGGAAGAATGCCACAATCCAAATAATATGAG 3388
HELP: FASTA Format
download the IRE sequence in FASTA format if you want to use it in sequence analysis programs as BLAST.Close
Overall Quality: High
- SIREs Prediction
- RNAfold Prediction
HELP: SIRES Prediction/RNAFold Prediction
Predicted pairings are graphically displayed using our algorithm and the RNAFold predicted program (Vienna RNA Package)
Close
Report
Click on the features to learn what they mean
SIREs Prediction
Loop is of the canonical type. Motif class: 1
HELP: Motif type
There are 18 types of motives. Motifs 1 and 2 are the canonical ones, while 3 to 18 are derived from SELEX experiments. Please look at the "Docs Pages" to know more CloseNo Mismatches
HELP: Mismatch
We allow one, but only one mistmatch in the stem. The presence of a mismatch an a bulge (see the Bulge help) are incompatibleClose-
No 3' Bulges
HELP: 3' Bulge
An unpaired base (a "Bulge") is allowed at positions 20b,21b,22b or 23b. The presence of a mismatch (see the mismatches help) and a buldge are incompatibleClose -
n25 is not a G
HELP: The 25th base
A G Residue in this position could compromise the IRE stability since it could pair with the C8, which needs to be free to interact with the Iron Regulatory proteins (IRPs).Close No GU Pairs
HELP: GU/UG Pairings
GU/UG parings are possible in RNA strands. However, experiments suggest that only 2 of those can be present in the IRE stem for it to be stable.CloseSIREs Prediction vs. RNAFold Prediction
-
Loop matches the RNAFold prediction
HELP: Predicted Loop
The 6 nucleotide apical loop is also fold as a loop in the RNAFold prediction. Close -
Pairings in the upper stem match the RNAFold prediction
HELP: Predicted Pairings
The base pairings in the upper stem predicted by SIREs are also pairing in the RNAFold prediction. Close C8 Bulge is present in the RNAFold prediction
HELP: C8 in the predicted folding
The C8 bulge predicted by SIREs is also predicted in the RNAFold structure as an unpair nucleotide. Nucleotide at position 8 (C8 bulge) is an important contact site with the IRPs. CloseFree Energy
Free Energy: -8.40 kcal/mol
HELP:Minimum Free Energy
Minimum Free Energy of the folded RNA structure has been calculated with the Vienna Package. Please visit Vienna package Site for further information.Close
Hover over the features to highlight them in the structure
Sequence ID
mouse-TFR1-NM_011638
Sequence Context
Position in sequence: 3602-3633
3468 TGTGGCCAAGTTAAATGTCATTTAAAGGCTATGGTAGTACTTACATCTACAAAATTTTAC 3527
3528 AGCTCAATTTATTCAAGATGTAACTCAAAATCAATTTTGCAAAATTTCCAGTACCTTTGT 3587
3588 CACAAACTTAACTCACATTATCGGGAGCAGTGTCTTCCATAATGCATAAAGAACAAGGTA 3647
3648 GTTTTTGCCTACCACAGTGTCTATATCGGAGACAGTGATCTCCATATGTTACACTAAGGG 3707
3708 TGTACGTAATTATCGGGAACAGTGTTTCCCATAATTTTCTTCATGCAATGACATCTTCAG 3767
HELP: FASTA Format
download the IRE sequence in FASTA format if you want to use it in sequence analysis programs as BLAST.Close
Overall Quality: High
- SIREs Prediction
- RNAfold Prediction
HELP: SIRES Prediction/RNAFold Prediction
Predicted pairings are graphically displayed using our algorithm and the RNAFold predicted program (Vienna RNA Package)
Close
Report
Click on the features to learn what they mean
SIREs Prediction
Loop is of the canonical type. Motif class: 1
HELP: Motif type
There are 18 types of motives. Motifs 1 and 2 are the canonical ones, while 3 to 18 are derived from SELEX experiments. Please look at the "Docs Pages" to know more CloseNo Mismatches
HELP: Mismatch
We allow one, but only one mistmatch in the stem. The presence of a mismatch an a bulge (see the Bulge help) are incompatibleClose-
No 3' Bulges
HELP: 3' Bulge
An unpaired base (a "Bulge") is allowed at positions 20b,21b,22b or 23b. The presence of a mismatch (see the mismatches help) and a buldge are incompatibleClose -
n25 is not a G
HELP: The 25th base
A G Residue in this position could compromise the IRE stability since it could pair with the C8, which needs to be free to interact with the Iron Regulatory proteins (IRPs).Close 1 GU Pair
HELP: GU/UG Pairings
GU/UG parings are possible in RNA strands. However, experiments suggest that only 2 of those can be present in the IRE stem for it to be stable.CloseSIREs Prediction vs. RNAFold Prediction
-
Loop matches the RNAFold prediction
HELP: Predicted Loop
The 6 nucleotide apical loop is also fold as a loop in the RNAFold prediction. Close -
Pairings in the upper stem match the RNAFold prediction
HELP: Predicted Pairings
The base pairings in the upper stem predicted by SIREs are also pairing in the RNAFold prediction. Close C8 Bulge is present in the RNAFold prediction
HELP: C8 in the predicted folding
The C8 bulge predicted by SIREs is also predicted in the RNAFold structure as an unpair nucleotide. Nucleotide at position 8 (C8 bulge) is an important contact site with the IRPs. CloseFree Energy
Free Energy: -9.00 kcal/mol
HELP:Minimum Free Energy
Minimum Free Energy of the folded RNA structure has been calculated with the Vienna Package. Please visit Vienna package Site for further information.Close
Hover over the features to highlight them in the structure
Sequence ID
mouse-TFR1-NM_011638
Sequence Context
Position in sequence: 3667-3698
3533 AATTTATTCAAGATGTAACTCAAAATCAATTTTGCAAAATTTCCAGTACCTTTGTCACAA 3592
3593 ACTTAACTCACATTATCGGGAGCAGTGTCTTCCATAATGCATAAAGAACAAGGTAGTTTT 3652
3653 TGCCTACCACAGTGTCTATATCGGAGACAGTGATCTCCATATGTTACACTAAGGGTGTAC 3712
3713 GTAATTATCGGGAACAGTGTTTCCCATAATTTTCTTCATGCAATGACATCTTCAGAGCTT 3772
3773 GAAGATCGTTAGTATCTAACATGAATTCCTAACTTCCTGTAGCTCCTTAGTTTTAGTTGC 3832
HELP: FASTA Format
download the IRE sequence in FASTA format if you want to use it in sequence analysis programs as BLAST.Close
Overall Quality: High
- SIREs Prediction
- RNAfold Prediction
HELP: SIRES Prediction/RNAFold Prediction
Predicted pairings are graphically displayed using our algorithm and the RNAFold predicted program (Vienna RNA Package)
Close
Report
Click on the features to learn what they mean
SIREs Prediction
Loop is of the canonical type. Motif class: 1
HELP: Motif type
There are 18 types of motives. Motifs 1 and 2 are the canonical ones, while 3 to 18 are derived from SELEX experiments. Please look at the "Docs Pages" to know more CloseNo Mismatches
HELP: Mismatch
We allow one, but only one mistmatch in the stem. The presence of a mismatch an a bulge (see the Bulge help) are incompatibleClose-
No 3' Bulges
HELP: 3' Bulge
An unpaired base (a "Bulge") is allowed at positions 20b,21b,22b or 23b. The presence of a mismatch (see the mismatches help) and a buldge are incompatibleClose -
n25 is not a G
HELP: The 25th base
A G Residue in this position could compromise the IRE stability since it could pair with the C8, which needs to be free to interact with the Iron Regulatory proteins (IRPs).Close No GU Pairs
HELP: GU/UG Pairings
GU/UG parings are possible in RNA strands. However, experiments suggest that only 2 of those can be present in the IRE stem for it to be stable.CloseSIREs Prediction vs. RNAFold Prediction
-
Loop matches the RNAFold prediction
HELP: Predicted Loop
The 6 nucleotide apical loop is also fold as a loop in the RNAFold prediction. Close -
Pairings in the upper stem match the RNAFold prediction
HELP: Predicted Pairings
The base pairings in the upper stem predicted by SIREs are also pairing in the RNAFold prediction. Close C8 Bulge is present in the RNAFold prediction
HELP: C8 in the predicted folding
The C8 bulge predicted by SIREs is also predicted in the RNAFold structure as an unpair nucleotide. Nucleotide at position 8 (C8 bulge) is an important contact site with the IRPs. CloseFree Energy
Free Energy: -7.90 kcal/mol
HELP:Minimum Free Energy
Minimum Free Energy of the folded RNA structure has been calculated with the Vienna Package. Please visit Vienna package Site for further information.Close
Hover over the features to highlight them in the structure
Sequence ID
mouse-TFR1-NM_011638
Sequence Context
Position in sequence: 3714-3745
3580 ACCTTTGTCACAAACTTAACTCACATTATCGGGAGCAGTGTCTTCCATAATGCATAAAGA 3639
3640 ACAAGGTAGTTTTTGCCTACCACAGTGTCTATATCGGAGACAGTGATCTCCATATGTTAC 3699
3700 ACTAAGGGTGTACGTAATTATCGGGAACAGTGTTTCCCATAATTTTCTTCATGCAATGAC 3759
3760 ATCTTCAGAGCTTGAAGATCGTTAGTATCTAACATGAATTCCTAACTTCCTGTAGCTCCT 3819
3820 TAGTTTTAGTTGCAAAAAACATTTGTGGTCATTGAGCATTTGGTGGGTAAAATCAACTGC 3879
HELP: FASTA Format
download the IRE sequence in FASTA format if you want to use it in sequence analysis programs as BLAST.Close
Overall Quality: High
- SIREs Prediction
- RNAfold Prediction
HELP: SIRES Prediction/RNAFold Prediction
Predicted pairings are graphically displayed using our algorithm and the RNAFold predicted program (Vienna RNA Package)
Close
Report
Click on the features to learn what they mean
SIREs Prediction
Loop is of the canonical type. Motif class: 1
HELP: Motif type
There are 18 types of motives. Motifs 1 and 2 are the canonical ones, while 3 to 18 are derived from SELEX experiments. Please look at the "Docs Pages" to know more CloseNo Mismatches
HELP: Mismatch
We allow one, but only one mistmatch in the stem. The presence of a mismatch an a bulge (see the Bulge help) are incompatibleClose-
No 3' Bulges
HELP: 3' Bulge
An unpaired base (a "Bulge") is allowed at positions 20b,21b,22b or 23b. The presence of a mismatch (see the mismatches help) and a buldge are incompatibleClose -
n25 is not a G
HELP: The 25th base
A G Residue in this position could compromise the IRE stability since it could pair with the C8, which needs to be free to interact with the Iron Regulatory proteins (IRPs).Close No GU Pairs
HELP: GU/UG Pairings
GU/UG parings are possible in RNA strands. However, experiments suggest that only 2 of those can be present in the IRE stem for it to be stable.CloseSIREs Prediction vs. RNAFold Prediction
-
Loop matches the RNAFold prediction
HELP: Predicted Loop
The 6 nucleotide apical loop is also fold as a loop in the RNAFold prediction. Close -
Pairings in the upper stem match the RNAFold prediction
HELP: Predicted Pairings
The base pairings in the upper stem predicted by SIREs are also pairing in the RNAFold prediction. Close C8 Bulge is present in the RNAFold prediction
HELP: C8 in the predicted folding
The C8 bulge predicted by SIREs is also predicted in the RNAFold structure as an unpair nucleotide. Nucleotide at position 8 (C8 bulge) is an important contact site with the IRPs. CloseFree Energy
Free Energy: -8.10 kcal/mol
HELP:Minimum Free Energy
Minimum Free Energy of the folded RNA structure has been calculated with the Vienna Package. Please visit Vienna package Site for further information.Close
Hover over the features to highlight them in the structure
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